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KMID : 0615720000170010251
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Jouranl of Korean Acupuncture and Moxibustion society
2000 Volume.17 No. 1 p.251 ~ p.286
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Study on the Anti-cancer , Anti-metastasis and Immune response improvement of Aqua-acupuncture with Cistanches Herba infusion solution
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Abstract
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To study the effects of anti-cancer, anti-metastasis and immune response improvement of aqua-acupuncture with Cistanches Herba infusion solution, we used Cistanches Herba infusion solution(taken by water-alcohol method) put into Chung-wan(CV12) and Chok-Samni(S36) of BALB/c or C57BL/6 which are corresponding to human body.
We observed the cytotoxicity, the effect on the expression of MMP-9 gene, the ability to control cancer cell proliferation, change of body weight, surviving number, MST, ILS, changes in amount of WBC, RBC, PLT, total protein, creatinine, glucose and LDH, weight of spleen, number of pulmonary colony, histological analysis on tissue metastasis of lung and liver, splenic cell proliferation, the expression of cytokine gene, the number of CD4^+, CD8^+, CD19^+ and NK cell, and concluded like this.
The results were obtained as follows :
1. The cytotoxicity about B16-F10 cell line of 2^0, 2^(-1), 2^(-2), 2^(-5) diluent groups in Cistanches Herba infusion solution treatment was inhibited significandy, compared with control group.
2. The cytotoxicity about HT1080 cell line of 2^0, 2^(-1), 2^(-2), 2^(-3), 2^(-5), 2^(-8) diluent groups in Cistanches Herba infusion solution treatment was inhibited significantly, compared with control group.
3. The effect on expression of MMP-9 gene was decreased in all the sample groups, compared with control group.
4. The effect on the control-ability on the cancer cell proliferation showed cytotooicity significantly in 2^0, 2^(-1), 2^(-2), 2^(-3), 2^(-4), 2^(-5) diluent groups.
5. S-180 cancer cell line transplants in BALB/c mice were inhibited significantly in weight increase in all the sample groups, compared with control group. The surviving number increased in almost sample groups, except one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group that showed same number of the control group.
6. S-180 cancer cell line transplants in BALB/c mice showed high MST and ILS significantly in almost sample groups, compared with control group. But one group put into Chok-Samni(S36) with 20% Cistanches Herba infusion solution treatment group showed low MST and ILS than control group.
7. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice showed increased ILS compared with control group significantly in anti-metastasis test.
8. The sample group injected in vein with B16-F10 cancer cell line in C57BL/6 mice were increased significantly in the number of WBC and glucose, and decreased significantly in the amount of LDH, compared with control group. However, there´s no significant increase or decrease in number of RBC, PLT, total protein and creatinine.
9. We couldn´t find any significant relation in spleen weight of the sample group.
10. In pulmonary colony, sample group was decreased significantly, compared with control group.
11. Histological analysis of sample group inhivited compared with that of control group in both of lung and liver.
12. In immune system, all the sample groups showed having more relevancy to the effect on splenic cell proliferation than normal group.
13. The effect on cytokine gene expression of all the sample groups were increased than control group.
14. In flow cytometry there´s no significant relation in number of CD8^+, CD19^+ cell, however, the number of CD4^+ cell and NK cell in sample groups were increased than in control group.
Above the results showed that aqua-acupuncture of Cistanches Herba infusion solution has effects of anti-cancer, anti-metastasis and immune response improvement.
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